Sequence analysis of Escherichia coli O157:H7 bacteriophage ΦV10 and identification of a phage-encoded immunity protein that modifies the O157 antigen |
| |
| Authors: | Lynda L. Perry,Phillip SanMiguel,Udit Minocha,Anton I. Terekhov,Mindy L. Shroyer,Leigh A. Farris,Nathan Bright,Bradley L. Reuhs,& Bruce M. Applegate |
| |
| Affiliation: | Food Science Department, Purdue University, West Lafayette, IN, USA;and;Department of Biological Sciences, Purdue University, West Lafayette, IN, USA |
| |
| Abstract: | Bacteriophage ΦV10 is a temperate phage, which specifically infects Escherichia coli O157:H7. The nucleotide sequence of the ΦV10 genome is 39 104 bp long and contains 55 predicted genes. ΦV10 is closely related to two previously sequenced phages, the Salmonella enterica serovar Anatum (Group E1) phage ɛ15 and a prophage from E. coli APEC O1. The attachment site of ΦV10, like those of its two closest relatives, overlaps the 3' end of guaA in the host chromosome. ΦV10 encodes an O -acetyltransferase, which modifies the O157 antigen. This modification is sufficient to block ΦV10 superinfection, indicating that the O157 antigen is most likely the ΦV10 receptor. |
| |
| Keywords: | Escherichia coli O157:H7 bacteriophage O antigen |
|
|
