Multilocus sequence analysis of Bacillus thuringiensis serovars navarrensis, bolivia and vazensis and Bacillus weihenstephanensis reveals a common phylogeny |
| |
Authors: | Brahim Soufiane Mathilde Baizet Jean-Charles Côté |
| |
Institution: | 1. Agriculture and Agri-Food Canada, Research Centre, 430 Gouin Blvd, Saint-Jean-sur-Richelieu, QC, J3B 3E6, Canada 2. Département de Chimie-Biologie, Université du Québec à Trois-Rivières, 3351 Boul. des Forges, P.O. Box 500, Trois-Rivières, QC, G9A 5H7, Canada
|
| |
Abstract: | The Bacillus cereus group sensu lato includes six closely-related bacterial species: Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides and Bacillus weihenstephanensis. B. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. B. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16S rRNA gene and cspA genes. A total of seven housekeeping genes (glpF, gmK, ilvD, pta, purH, pycA and tpi) from different B. thuringiensis serovars and B. weihenstephanensis strains were amplified and their nucleotide sequences determined. A maximum likelihood phylogenetic tree was inferred from comparisons of the concatenated sequences. B. thuringiensis serovars navarrensis, bolivia and vazensis clustered not with the other B. thuringiensis serovars but rather with the B. weihenstephanensis strains, indicative of a common phylogeny. In addition, specific signature sequences and single nucleotide polymorphisms common to B. thuringiensis serovars navarrensis, bolivia and vazensis and the B. weihenstephanensis strains, and absent in the other B. thuringiensis serovars, were identified. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|