Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources |
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Authors: | M Vuleti? V Had?i-Ta?kovi? ?ukalovi? K Markovi? J Dragi?i? Maksimovi? |
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Institution: | (1) Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India;(2) Biochemistry/Bioinformatics Research Lab, Department of Zoology, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, 221005, India |
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Abstract: | The activities of antioxidative enzymes and contents of proline and total phenolics were assayed in roots of two maize (Zea mays L.) genotypes grown in a medium containing nitrate (NO3
−) or both nitrogen forms, nitrate and ammonium (NH4
+/NO3
−). An increase in the activities of class III peroxidases (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), ascorbate
oxidase (AO) and proline content, and decrease in phenolic content were observed in NH4
+/NO3
− in comparison with NO3
− grown plants. When polyethylene glycol (PEG) was added to both nitrogen treatments, the content of total phenolics and proline
was increased, especially in NH4
+/NO3
− treatment. The PEG treatment decreased enzyme activities in NH4
+/NO3
− grown plants, but in NO3
− grown plants activities of POD and SOD were increased, opposite to decreased APX and AO. Isoelectric focusing demonstrated
increased activities of acidic POD isoforms in PEG treated NO3
− grown plants, and lower activities of both, acidic and basic isoforms in NH4
+/NO3
−grown plants. |
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Keywords: | |
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