Cytokinins,auxins and activated charcoal affect organogenesis and anatomical characteristics of shoot-tip cultures of lisianthus [<Emphasis Type="Italic">Eustoma grandiflorum</Emphasis> (RAF.) Shinn] |
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Authors: | Email author" target="_blank">Kee-Yoeup?PaekEmail author Eun-joo?Hahn |
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Institution: | (1) Department of Horticulture, Chungbuk National University, Cheongju, 361-763 Chungbuk, South Korea;(2) Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763 Chungbuk, South Korea |
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Abstract: | Summary Variants from seed-propagated Lisianthus Eustoma grandiflorum (Raf.) Shinn] were shoot-tip cultured to observe the effects of cytokinins, auxins and activated charcoal on organogenesis
and anatomical characteristics. N6-Benzyladenine (BA) and kinetin at high concentrations (13.32–22.2 and 13.94–23.23 μM) resulted in good shoot formation but high percentages of hyperhydric shoots. Increased indole-3-acetic acid (IAA) and indole-3-butyric
acid (IBA) concentrations favored root formation, while increased naphthaleneacetic acid concentration adversely affected
root formation. Both shoot and root development were suppressed by activated charcoal. The highest percentage of regeneration
and the largest number of glaucous shoots with an average of 15 shoots per explant after 4 wk of culture were obtained when
the shoot tips were cultured on MS (Murashige and Skoog, 1962) medium supplemented with 4.44 μM BA and 1.47–4.92 μMIAA and IBA. In vitro-grown leaves had a higher number of stomata than field-grown leaves but the length and diameter of stomata showed no significant
difference between the two types. Field-grown leaves had well-developed epicuticular wax layers. which were not observed on
hyperhydric leaves. Hyperthydric plantlets could not survive when transplanted to soil, whereas glaucous plantlets survived
in more than 80% of cases. Variation in soil type resulted in a slight difference in plantlet survival. Based on the results
of our experiment, this protocol should be useful for the rapid micropropagation of lisianthus. |
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Keywords: | Eustonia grandiflorum hyperhydration lisianthus scanning electron microscopy variants |
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