Genetic diversity of <Emphasis Type="Italic">Pistacia khinjuk</Emphasis> Stocks. using RAPD markers and leaf morphological characters |
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Authors: | Hamid Reza Karimi Mahbubeh Hajizadeh Hossin Abadi Azam Maleki Kohbanani |
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Institution: | (1) Department of Horticultural Science, College of Agriculture, Vali-e-Asr University, Rafsanjan, Kerman, Iran |
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Abstract: | The aim of this research was to study the genetic diversity of Pistacia khinjuk Stocks. and also to reveal the genetic relationships. Leaf morphological characters of 16 genotypes together with Randomly
Amplified Polymorphic DNA (RAPD) marker data were used for this purpose. Leaf morphological characters were used for factor
analysis, which determined four main factors. Grouping of genotypes by these factors was performed by Ward’s method. Fifteen
primers produced a total of 146 fragments, with an average of 9.73 fragments per primer, of which 129 were polymorphic. The
unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on Jaccard’s similarity coefficient
matrix. According to RAPD data, genotypes were separated into three groups. The first contained the genotypes with round leaflets.
Genotypes with three lanceolate leaflets were located in the second and third groups, respectively. This study revealed that
high diversity exists among genotypes of P. khinjuk, like in P. vera. Also it could be postulated that genotypes of P. khinjuk with oblique and lanceolate leaflets are probably descendents of the genotypes with acuminate and roundish leaflets. |
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