Establishing two-dimensional gels for the analysis of Leishmania proteomes |
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| Authors: | Acestor Nathalie Masina Slavica Walker John Saravia Nancy G Fasel Nicolas Quadroni Manfredo |
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| Affiliation: | Institute of Biochemistry, University of Lausanne, Switzerland. |
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| Abstract: | Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility. |
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