Shikimate pathway activity in shake and fermenter cultures of Cinchona succirubra |
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Authors: | H -P Schmauder D Gröger H Koblitz D Koblitz |
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Institution: | (1) Institut für Biochemie der Pflanzen, Akademie der Wissenschaften der DDR, DDR-4020 Halle/Saale;(2) Zentralinstitut für Genetik und Kulturpflanzenforschung, Akademie der Wissenschaften der DDR, DDR-4325 Gatersleben |
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Abstract: | Cell suspension cultures of Cinchona succirubra were cultivated in shake cultures and for the first time in airlift fermenters. Under both conditions L-tryptophan exerts a stimulatory effect on alkaloid formation. In this context the regulatory pattern of some shikimate pathway enzymes was investigated in non-supplemented and tryptophan supplemented Cinchona cell cultures. A remarkable increase of tryptophan decarboxylase (TDC) activity was observed in Cinchona cells under the influence of tryptophan. Apparently, like in some other indole alkaloid producing cell cultures, a high TDC activity is a prerequisite for alkaloid formation. Growth pattern and some enzyme activities of C. succirubra fermenter cultures at controlled and non-regulated pH levels were followed. Optimum growth and alkaloid formation were recorded under non-regulated (normal) pH conditions.Abbreviations TDC
tryptophan decarboxylase
- try
L-tyrosine
- phe
L-phenylalanine
- DAHP
3-deoxy-D-arabino-heptulosonic acid-7-phosphate
- trp
L-tryptophan
- E-4-P
erythrose-4-phosphate
- PEP
phosphoenolpyruvate
- MDH
malate dehydrogenase
- G-6-PDH
glucose-6-phosphate dehydrogenase
- 6-PG-DH
6-phosphogluconate dehydrogenase
- Ch-mutase
chorismate mutase
- AS-synthase
anthranilate synthase
- n.d.
not determined |
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