Genetic analysis of plasmid determinants for microcin J25 production and immunity. |
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| Authors: | J O Solbiati M Ciaccio R N Farías and R A Salomn |
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| Institution: | J O Solbiati, M Ciaccio, R N Farías, and R A Salomón |
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| Abstract: | Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical analysis of insertion mutations and complementation tests defined three contiguous genes involved in MccJ25 production which span a region of about 2.2 kb. Immunity to the antibiotic is provided by an additional gene adjacent to the production region. |
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