Microphytobenthic biomass assessment by pigment analysis: comparison of spectrophotometry and High Performance Liquid Chromatography methods |
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Authors: | Vanda Brotas Carlos Rafel Mendes Paulo Cartaxana |
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Institution: | (1) Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Campo Grande, 1749-016 Lisbon, Portugal |
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Abstract: | Samples of microphytobenthos from the Tagus estuary were analysed for photosynthetic pigments by spectrophotometry and High
Performance Liquid Chromatography (HPLC). Chlorophyll a values obtained with HPLC and spectrophotometry methods presented a highly significant positive correlation for both spectrophotometric
methods used (with and without the correction for pheopigments), but this relationship depended on the type of sediment. We
concluded that spectrophotometric methods give reliable Chl-a values, being suited for routine analysis, when a vast number of replicates is needed. However, for the correct estimation
of pheopigments, HPLC analysis is indispensable. In the literature, Chl-a estimations are expressed per content (μg g−1) or concentration (mg m−2). We discuss the influence of sediment type on the results depending on the type of unit used, and propose a simple conversion
factor based on sediment water content. |
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Keywords: | Microphytobenthos Biomass Chlorophyll a HPLC Spectrophotometry Water content |
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