Cloning and expression pattern of odorant receptor 11 in Asian honeybee drones,Apis cerana (Hymenoptera,Apidae) |
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Authors: | Jun Feng Liu Le Yang Mang Li Xu Jiang He Zi Long Wang Zhi Jiang Zeng |
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Institution: | 1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, PR China;2. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, PR China;3. Kaihua County Animal Husbandry and Veterinary Bureau, Quzhou 324300, PR China |
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Abstract: | Odorant receptors play a crucial role in the special recognition of scent molecules in the honeybee olfaction system. The odorant receptor 11 (AmOR11) in western honeybee drones (Apis mellifera) has been demonstrated to specifically bind to 9-oxo-2-decenoic acid (9-ODA) of queens. However, little is known regarding the functions of OR11 Asian honeybee drones (Apis cerana) in the context of their mating activities. In this study, the odorant receptor 11 gene (AcOr11) from A. cerana was cloned, and its expression profiles were examined during two developmental stages (immature and sexually mature) and different physiological statuses (flying and crawling). The cDNA sequence of AcOr11 was highly similar to that of AmOr11, and encoded a membrane-coupled protein of 384 amino acids. The results of qRT-PCR indicated that AcOr11 was expressed at higher levels in drone antennae compared to brains, and the expression was significantly up-regulated in sexually mature drone brains compared to immature brains. Interestingly, AcOr11 expression in brains of mature flying drones was dramatically higher than those of mature crawling drones. To our knowledge, this study demonstrate a link between AcOr11 gene expression in the brain of honeybee drones and behavior associated with sexual maturity and mating flight. |
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Keywords: | Corresponding author Odorant receptor 11 Drones Mating flight Sexual development |
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