Orexin-A受体介导的生长抑素激动剂ODT8-SST对大鼠摄食和饮水的影响

目的：探讨orexin-A（OXA）受体介导的生长抑素激动剂ODT8-SST 对大鼠摄食和饮水的调节作用相关作用机制。方法：在光 照周期内，大鼠40 只随机分8 组，侧脑室（icv）分别注射不同剂量ODT8-SST 或生理盐水（NS）；大鼠56 只随机分8 组分别侧脑 室注射不同剂量OXA 受体（OX1R）拮抗剂SB-334867 或NS；2小时后测量大鼠摄食量和饮水量。结果：与NS组相比，实验组大 鼠侧脑室注射ODT8-SST（1 ug/rat），2 小时后摄食量和饮水量均显著增加（P<0.05）。大鼠侧脑室注射SB-334867（16 ug/rat）完全 抑制了由侧脑室注射ODT8-SST 后引起的摄食量和饮水量的增加；与此相反，大鼠给予SST2 拮抗剂S-406-028 预处理之后，可阻 止侧脑室注射ODT8-SST 引发的促进食欲作用，但不会影响侧脑室注射OXA（10.7 ug/rat）诱导的摄食量和饮水量的增加。结论： 侧脑室注射ODT8-SST 可促进摄食和饮水，该过程可能由OX1R所介导；orexin-A 促进摄食作用不依赖大脑SST2 通路的激活。

Effect of Orexin-A Receptor Mediated Somatostatin Agonists ODT8-SST of Rats on Food Intake and Water Intake

Objective:To investigate the orexin-A (OXA) receptor mediated somatostatin agonists ODT8-SST on the regulation of food intake and water intake of rats and OXA promoting feeding mechanism.Methods:In the light period, 40 rats were randomly divided into 8 groups, the lateral ventricle (icv) were injected with different doses of ODT8-SST or normal saline (NS); 56 rats were randomly divided into 8 groups, icv were injected with different doses of OXA receptor (OX1R) antagonist SB-334867 or NS; the food intake and water intake of rat were recorded for 2 hours after the injection.Results:Compared with NS control group, after injecting ODT8-SST (1 ug / rat, icv) into ICV, the food intake and water intake of experimental group were significantly increased (P <0.05). Lateral ventricle injection of OXA receptor (OX1R) antagonist SB-334867 (16 ug / rat) completely inhibited the increase in food intake and water intake caused by lateral ventricle injection of ODT8-SST; with in contrast, rats were given SST2 antagonist S-406-028 after pretreatment, can prevent the promotion of the role of appetite caused by intracerebroventricular injection of ODT8-SST, but does not affect the lateral ventricle injection OXA (10.7 ug/rat) induced food intake and water intake increased.Conclusion:Intracerebroventricular injection of ODT8-SST can promote feeding and drinking, OX1R signaling pathway is a part of the neural circuits; orexin-A promotes the ingestion does not depend on the activation of the brain SST2 pathway.