担载b-FGF的PLGA微球复合明胶支架的体外释放研究

目的:研究担载碱性成纤维细胞生长因子(b-FGF)微球复合明胶支架的外形特征、孔径、孔隙率及体外释放动力学,以期构建具有缓释功能、高孔隙率的担载细胞因子的新型复合明胶支架。方法:本文利用冷冻相分离法和S/O/W法先将b-FGF水溶液包裹于PLGA微球中,然后埋置于明胶溶液中制备为多孔复合明胶支架。分别对微球的形态和复合明胶支架的基本形态、孔径、孔隙率进行表征,通过Elisa法测定b-FGF在复合明胶支架中的体外释放行为。结果:制备成形态良好的三维复合明胶支架,其孔隙率为82.90%±1.45%,孔径范围为150~300μm,复合明胶支架中b-FGF在体外缓慢释放20余天。结论:担载蛋白微球复合明胶支架不仅满足组织工程支架的要求,还能有效缓释细胞因子,为细胞和组织生长提供良好的微环境,为进一步应用于组织工程领域提供了可能。

In Vitro Release Research of Gelatin Scaffold with PLGA Microspheres Containing Basic Fibroblast Growth Factor

Objective:To investigate the characterization, the pore size and the porosity ratio of gelatin scaffold with PLGA microspheres containing basic fibroblast growth factor and its release behavior in vitro of b-FGF, for constructing a new composite gelatin scaffold loaded growth factor with sustain-released and high porosity ratio.Methods:By the frozen phase separation method and S/O/W method b-FGF aqueous solution prepared into PLGA microspheres, then embedded into gelatin-scaffold to formthe final composite scaffold. The morphology of microspheres and gelatin scaffold, the pore size and the porosity ratio of gelatin scaffold were characterized and release kinetic of b-FGF in composite gelatin scaffold was detected by Elisa method in vitro.Results:The composite three-dimensional (3D) scaffold had good morphology with the porosity ratio of 82.90%± 1.45%and the pore sizes ranging from 150 to 300 um. In vitro, b-FGF in composite gelatin scaffold had sustained release over 20 days.Conclusion:The gelatin scaffold with PLGA microspheres containing growth factor is not only satisfied the requirements of tissue engineering scaffolds, but also sustained release growth factor for providing a microenvironment to cells and tissue growth and further application to tissue engineering in future.