模拟室内高浓度PM2.5对大鼠血清和组织VEGF蛋白表达的影响

目的:采用"密闭环境熏烟法"模拟PM2.5高浓度环境,建立大鼠被动吸烟模型,观察模拟公共场所室内高浓度PM2.5对正常大鼠血清血管内皮生长因子(VEGF)以及心、脾、肺三种组织VEGF蛋白表达的影响。方法:将20只6月龄雄性健康Wistar大鼠按体重分层随机分为实验组和对照组。并且两组大鼠均于相同的环境下(温度,湿度,光照)进行饲养。对照组不做任何处理,实验组进行为期6周的密闭环境烟熏。末次烟熏结束24-36小时内,用ELISA检测两组大鼠血清VEGF水平的变化,用Western blot检测心、脾、肺三种组织VEGF蛋白表达的变化。结果:与对照组比较,实验组心、脾VEGF1、2以及肺VEGF1蛋白表达水平均显著下降,而肺VEGF2和血清VEGF水平无显著变化。结论:6周模拟公共场所室内高浓度PM2.5能显著降低大鼠心、脾、肺VEGF蛋白表达水平。

Effects of High Concentrations of PM2.5 in Simulated Indoor Public Places on the Serum Level of VEGF and the Protein Expression of VEGF in Heart, Spleen, and Lung Tissues of Rats

Objective:To establish passive smoking rat model by simulating the high concentration of PM2.5 in indoor public places, the changes of serum level of VEGF and the protein expression of VEGF in heart, spleen, lung tissues of rats were observed in this paper.Methods:20 healthy male Wistar 6-month-old rats were randomly divided into two groups, experimental group and control group. And two groups of rats were feed in the same environment (temperature, humidity, illumination). The experimental group rats were exposed to passive cigarette smoking 2 hours every day in closed environment for 6 weeks. After 24-36 h of the final treatment, the protein expression of VEGF in heart, spleen, lung tissues were detected by Western blot and the serum level of VEGF was detected by ELISA.Results:The protein levels of VEGF1, 2 in heart and spleen and VEGF1 in lung of experimental group were significantly lower than that in control group. However, the VEGF2 protein expression in lung and the serum level of VEGF had no significant differences.Conclusion:The high concentrations of PM2.5 in simulated indoor public places can reduce the protein expression of VEGF in heart, spleen and lung of rats.