The in vitro synthesis and secretion of alpha-ecdysone by the ring glands of the fly, Sarcophaga bullata. |
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Authors: | W E Bollenbacher W Goodman W V Vedeckis L I Gilbert |
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Institution: | Department of Biological Sciences Northwestern University Evanston, Illinois 60201 USA |
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Abstract: | The in vitro secretory product of larval Sarcophage bullata ring glands has been identified as 2beta, 3beta, 14alpha, 22R, 25-pentahydroxy-5beta-cholest-7-en-6-one (alpha-ecdysone). Mid to late 3rd instar larval ecdysones were isolated and identified as 2beta, 3beta, 14alpha, 20R, 22R, 25-hexahydroxy-5beta-cholest-7-en-6-one (beta-ecdysone) and alpha-ecdysone at a ratio of 27:1. The low level of alpha-ecdysone in vivo, relative to its exclusive in vitro synthesis and secretion by the ring glands, is a function of the very active C20 hydroxylation mechanism in tissues peripheral to the ring gland. The role of alpha-ecdysone as a prohormone in dipteran metamorphosis is discussed. |
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