Photomorphogenic effects on in vitro rooting of Prunus roostock GF 655-2 |
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Authors: | Federica Rossi Rita Baraldi Osvaldo Facini Bartolomeo Lereari |
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Institution: | (1) Centro di Studio per la Tecnica Frutticola, Consiglio Nazionale delle Ricerche, via Filippo Re 6, 40126 Bologna, Italy;(2) Dipartimento di Biologia delle Piante Agrarie, viale delle Piagge 26, 56100 Pisa, Italy |
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Abstract: | The morphogenic effect of different light wavelengths on in vitro rooting of Prunus insititia GF655-2 in relation to the presence of napthaleneacetic acid (NAA) in the culture medium was investigated. Results of experiments in which plantlets were rooted in NAA enriched medium showed that the presence of auxin induced rooting even in the dark after an initial lag period. Illumination of the cultures with Red light was as effective in promoting rooting as treatment with 0.5 M NAA; Red was more active in stimulating rooting in the short term than was NAA. The pattern of root formation resulting from the addition of NAA appeared to dominate development under White, Blue and Far Red treatments. Although it was possible to correlate the rooting response to the phytochrome photoequilibrium induced by the light treatments used, there arises a possible interference of specific Blue absorbing photoreceptors.Abbreviations B
Blue
- FR
Far Red
- HIR
High Irradiance Response
- Pfr
active (far-red absorbing) form of phytochrome
- Ptot
total phytochrome
- R
Red
- W
White
- NAA
-naphtaleneacetic acid
- BA
benzyladenine
- IAA
indole 3-acetic acid |
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Keywords: | blue light photoreceptors light quality micropropagation NAA phytochrome rhizogenesis |
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