The Salmonella typhimurium glycine cleavage enzyme system |
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Authors: | George V Stauffer Lorraine T Stauffer and Michael D Plamann |
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Institution: | (1) Department of Microbiology, University of Iowa, 52242 Iowa City, IA, USA;(2) Present address: Department of Biological Sciences, Stanford University, 94305 Stanford, CA, USA |
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Abstract: | Summary A glycine cleavage enzyme system, inducible by glycine, has been demonstrated in Salmonella typhimurium. The induced enzyme levels, however, are only about 20% of the induced levels found in Escherichia coli. Starting with a serine auxotroph, mutants were isolated that grow with a serine supplement, but not with a glycine supplement. Three independently isolated mutants have reduced or nondetectable glycine cleavage enzyme levels. The new mutations, designated gcv, were mapped between the serA and lys genes at 62.5 min on the S. typhimurium chromosome.Abbreviations C1
one-carbon
- GCV
glycine cleavage
- GM
glucose minimal
- L agar
Luria agar
- LB
Luria broth
- Tc
tetracycline |
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Keywords: | gcv Glycine cleavage Regulation Mutations |
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