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Changes in isoperoxidases during shoot formation in tobacco callus
Authors:Thorpe  Trevor A.  Gaspar  Thomas
Affiliation:(1) Laboratoire de Biologie végétale, Institut Van Beneden, B-4020 Liège, Belgium;(2) Present address: Department of Biology, The University of Calgary, T2N 1N4 Calgary, Alberta, Canada
Abstract:Summary Shoot formation in tobacco (Nicotiana tabacum L.) callus is accompanied by an increase in peroxidase activity which takes a form similar to a sigmoid curve. The “stationary” phase coincide with the period of organ formation. Characteristic changes in isoperoxidase pattern are found in the shoot-forming part of the callus. These changes are different from those in the nonshoot-forming part or in gibberellin-treated tissue, which does not form shoots.
Keywords:isoperoxidases  starch gel electrophoresis  shoot formation  tobacco callus
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