Enhancement of salt tolerance in transgenic rice expressing an Escherichia coli catalase gene, katE |
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| Authors: | Kenji Nagamiya Tsuyoshi Motohashi Kimiko Nakao Shamsul Haque Prodhan Eriko Hattori Sakiko Hirose Kenjiro Ozawa Yasunobu Ohkawa Tetsuko Takabe Teruhiro Takabe Atsushi Komamine |
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| Institution: | (1) Tokyo University of Agriculture, 1737 Funako, Atsugi 243-0034, Japan;(2) National Institute of Agrobiological Science, 2-1-2 Kannondai, Tsukuba Ibaraki, 305-8602, Japan;(3) National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo Hokkaido, 062-8555, Japan;(4) Graduate School of Bioagricultural Science, Nagoya University, Furoucho, Chikusa-ku, Nagoya 646-0804, Japan;(5) Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya 468-8502, Japan;(6) The Research Institute of Evolutionary Biology, 2-4-28 Kamiyoga, Setagaya, Tokyo 158-0098, Japan;(7) Sugimura International Patent and Trade Mark Agency Bureau, Kazan Building 7th floor, 3-2-4 Kasumigaseki, Chiyoda-ku, Tokyo 100-0013, Japan |
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| Abstract: | Rice (Oryza sativa) is sensitive to salt stresses and cannot survive under low salt conditions, such as 50 mM NaCl. In an attempt to improve
salt tolerance of rice, we introduced katE, a catalase gene of Escherichia coli, into japonica rice cultivar, Nipponbare. The resultant transgenic rice plants constitutively expressing katE were able to grow for more than 14 days in the presence of 250 mM NaCl, and were able to form flower and produce seeds in
the presence of 100 mM NaCl. Catalase activity in the transgenic rice plants was 1.5- to 2.5-fold higher than non-transgenic
rice plants. Our results clearly indicate that simple genetic modification of rice to express E. coli-derived catalase can efficiently increase its tolerance against salt stresses. The transformant presented here is one of
the most salt-tolerant rice plants created by molecular breeding so far. |
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| Keywords: | Catalase katE Oryza sativa Salt tolerance |
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