Glutaredoxin 2a,a mitochondrial isoform,plays a protective role in a human cell line under serum deprivation |
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Authors: | Su-Jung Kim Hyun-Joo Jung Hojin Choi Chang-Jin Lim |
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Institution: | (1) Department of Biochemistry, College of Natural Sciences, Kangwon National University, 192-1 Hyoja-2-dong, Chuncheon, 200-701, Korea;(2) College of Pharmacy, Sookmyung Women’s University, 52 Hyochangwon-gil, Seoul, 140-742, Korea;(3) Department of Neurology, Hanyang University College of Medicine, 249-1 Gyomun-dong, Guri-city, 471-701, Korea; |
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Abstract: | The roles of mitochondrial glutaredoxin (Grx2a) under serum deprivation were assessed using the human stable HepG2 cell lines
overexpressing or down-regulating Grx2a. The Grx2a-overexpressing stable cells displayed enhanced proliferation, decreased
reactive oxygen species (ROS) and caspase-3 activity levels, and increased total GSH level, compared to the vector control
cells. These characteristics of the overexpressing stable cells were reversed by down-regulating Grx2a in the same cell line.
In the limited serum conditions, the Grx2a-overexpressing stable pcDNA3.0/HA-Grx2a cells exhibited higher cellular viabilities
and total GSH level, and showed much lower enhancement in ROS and caspase-3 activity levels than the vector control pcDNA3.0/HA
cells. However, the Grx2a-down-regulating stable cells gave rise to diminished cellular viabilities and further decreased
total GSH level, and contained significantly higher ROS and caspase-3 activity levels, under serum deprivation than the vector
control cells. These results suggest that Grx2a plays proliferative and anti-apoptotic roles under serum deprivation. |
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