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| 一株产丙烯酸和一株耐高浓度丙烯酸细菌的筛选与鉴定 | |
| 引用本文： | 陈云来,左文朴,杜丽琴,韦宇拓,黄日波.一株产丙烯酸和一株耐高浓度丙烯酸细菌的筛选与鉴定[J].生物加工过程,2008,6(6):63-66. |
| 作者姓名： | 陈云来 左文朴 杜丽琴 韦宇拓 黄日波 |
| 作者单位： | 1. 广西大学,生命科学与技术学院,南宁,530005 2. 广西大学,生命科学与技术学院,南宁,530005;广西亚热带生物资源保护利用重点实验室,南宁,530005 3. 广西大学,生命科学与技术学院,南宁,530005;广西亚热带生物资源保护利用重点实验室,南宁,530005;广西科学院,南宁,530003 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 从广西大学农场、养猪场排水沟、奶牛场的排水沟、屠宰场的废液池及鱼塘的污泥和废水中分离到1株能产丙烯酸和1株耐高浓度丙烯酸的菌株。经高效气相色谱检测其培养提取物，能产丙烯酸的菌株在培养物中丙烯酸的摩尔浓度达到3．5mmoL／L；耐高浓度丙烯酸的菌株其耐受丙烯酸的摩尔浓度可以达到2moL／L，对这2个菌株进行16S rDNA鉴定，它们分别属于Cellulosimicrobium属和Brevundimonas属。 |
| 关 键 词： | 丙烯酸 鉴定 筛选 |
Screening, identification and characterization of an acrylic acid-producing and a high-level acrylic acid tolerance strains |
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| CHEN Yun-lai,ZUO Wen-pu,DU Li-qin,WEI Yu-tuo,HUANG Ri-bo.Screening, identification and characterization of an acrylic acid-producing and a high-level acrylic acid tolerance strains[J].Chinese Journal of Bioprocess Engineering,2008,6(6):63-66. | |
| Authors: | CHEN Yun-lai ZUO Wen-pu DU Li-qin WEI Yu-tuo HUANG Ri-bo |
| Institution: | CHEN Yun-lai, ZUO Wen-pu, DU Li-qin, WEI Yu-tuo,HUANG Ri-bo(1. College of Life Science and Technology, Guangxi University, Nanning 530005, China;2. Guangxi Key Laboratory of Subtropical Bioresouree Conservation and Utilization, Guangxi University, Nanning 530005, China; 3. Guangxi Academy of Science, Nanning 530003, China) |
| Abstract: | An acrylic acid-producing and a high-level acrylic acid tolerance strains were isolated from a local farm land as well as a fish ponds of the farm. The strains were designated as 5# and 1 500#. By detecting the cell-free extracts of the gas chromatography, strain 5 # accumulated acrylic acid up to 3.5mmoL/L. Meanwhile, strain 1 500# tolerated high-level acrylic acid up to 2 mol/L. The 16S rDNA sequence of these two strains were identified. The results revealed that strains 5# and 1 500# were Cellulosimicrobium and Brevundimonas, respectively. |
| Keywords: | acrylic acid identification screening |
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