Isolation and identification of 23,25-dihydroxy-24-oxo-vitamin D3: A metabolite of vitamin D3期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Isolation and identification of 23,25-dihydroxy-24-oxo-vitamin D3: A metabolite of vitamin D3

Authors:	Eberhard Mayer GSatyanarayana Reddy Jay R Kruse George Popjak Anthony W Norman

Institution:	1. Department of Biochemistry, University of California, Riverside, CA 92521 USA;1. Children''s Hospital Research Foundation, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 USA;2. Department of Biological Chemistry, School of Medicine, University of California, Los Angeles, CA 90024 USA

Abstract:	A new metabolite of vitamin D₃ has been isolated in pure form from incubations of rat kidney homogenates with 25-hydroxyvitamin D₃ 25-OH-D₃]. It was identified as 23,25-dihydroxy-24-oxo-vitamin D₃ 23,25(OH)₂-24-oxo-D₃] by means of ultraviolet absorption spectrophotometry and mass spectrometry. Also, 25-OH-D₃-26,23-lactone and 24R,25-dihydroxyvitamin D₃ were obtained from the same incubation mixtures. The enzyme activity responsible for the conversion of 25-OH-D₃ to 23,25(OH)₂-24-oxo-D₃ was induced by perfusion of the kidneys $in vitro$ with 50 nM 1,25-dihydroxyvitamin D₃ 1,25(OH)₂D₃].

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