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Changes in intracellular location of DNA polymerase-α during oocyte maturation of the toad,bufo bufo japonicus |
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| Authors: | Hiroshi Nagano Keiju Okano Susumu Ikegami Chiaki Katagiri |
| Institution: | 1. Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Hongo, Bunkyoku, Tokyo 113, Japan;2. Department of Physiology, Teikyo University School of Medicine, Kaga, Itabashiku, Tokyo 173, Japan;3. Department of Applied Biochemistry, Hiroshima University, Fukuyama, Hiroshima 720, Japan;4. Zoological Institute, Faculty of Science, Hokkaido University, Sapporo 060, Japan |
| Abstract: | Intracellular location of DNA polymerase-α during oocyte maturation of the toad was studied. Quantitative and qualitative changes in the activity of DNA polymerase-α were not observed during the maturational process. Nearly all activity was found in isolated germinal vesicles from full grown oocytes and in enucleated mature oocytes. The cytoplasmic DNA polymerase-α of mature oocytes was recovered at buoyant densities equivalent to microsome by isopycnic centrifugation. These findings indicate that DNA polymerase-α in the germinal vesicle is released into the cytoplasm and binds to the endoplasmic reticulum when the germinal vesicle breaks down. |
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