Occurrence of reducing terminal N-acetylglucosamine 3-sulfate and fucosylated outer chains in acidic N-glycans of porcine zona pellucida glycoproteins |
| |
| Authors: | Etsuko Mori Jerry L Hedrick Nate J Wardrip Tsuneatsu Mori Seiichi Takasaki |
| |
| Institution: | (1) Department of Immunology, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-kuTokyo, 108, Japan;(2) The Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA;(3) Departments of Biochemistry, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-kuTokyo, 108, Japan |
| |
| Abstract: | Structures of acidic N-glycans released from porcine zona pellucida glycoproteins by hydrazinolysis were studied. The results indicated that the acidic glycans are of mono- to tetraantennary complex-type with and without N-acetyllactosamine repeating units. Sulfated residues are not only located at the C-6 position of GlcNAc included in the N-acetyllactosamine repeating units, but also at the C-6 position of GlcNAc in the non-repeated antennae and at the C-3 position of reducing terminal GlcNAc residue. Analysis of the oligosaccharide fragments released by endo- -galactosidase digestion and by hydrazine/nitrous acid treatment also revealed that various sulfated and non-sulfated forms of fucosylated structures such as Fuc 1 2Gal14(±SO–36)GlcNAc (type 2H), Gal14(Fuc13)(±SO–36)GlcNAc(Lex) and Fuc13 or 4(±SO–36)GlcNAc, are expressed in the repeated outer chain moieties. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
