The effect of phosphate concentration on growth and agar content of Gelidium robustum (Gelidiaceae,Rhodophyta) in culture |
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| Authors: | Sousa-Pinto Isabel Lewis Ray Polne-Füller Miriam |
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| Affiliation: | (1) Department of Biological Sciences and Marine Sciences Institute, University of California, 93106 Santa Barbara, Santa Barbara, CA, USA;(2) Present address: Instituto Botânico Dr. Gonçalo Sampaio, Faculdade de Ciências da Universidade do Porto. R. do Campo Alegre, 1191, 4150 Porto, Portugal;(3) Present address: Department of Forestry, Fisheries & Wildlife, University of Nebraska, 68583-0814 Lincoln, NE, USA |
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| Abstract: | Phosphate concentration of the growth medium was found to affect the growth rate and agar yield of a clone of Gelidium robustum grown in the laboratory. To study differences in growth we used phosphate concentrations from 0 to 200 µM. To determine the effect of phosphate on agar yield and its properties we used concentrations from 0 to 20 µM. Growth rates generally increased with increasing phosphate concentration, with the highest growth rate (21% d–1) obtained at 150 µM. Agar yield as percentage of fresh weight was highest (10%) in the algae grown with low phosphate concentrations, but agar yield as percentage of dry weight was highest(43%) at 20 µM of phosphate. Gel strength increased with phosphate concentration with a maximum of 160 g m–2 for 0.75% gels for the cultures at 20 µM. Melting and gelling temperatures of the gels were also affected by phosphate concentration of the growth medium. Starch yield was highest in algae grown in low phosphate concentrations. |
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| Keywords: | agar agarophyte Gelidium phosphate Rhodophyta seaweed |
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