Histochemical study of glycoconjugates in the epididymis of the hamster (Mesocricetus auratus) |
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Authors: | Alfonso Calvo Luis M Pastor Ramon Horn and Jacinto Pallares |
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Institution: | (1) Department of Cellular Biology, Section of Histology and General Embryology, Medical School, University of Murcia, 30071 Murcia, Spain |
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Abstract: | Summary The glycoconjugates of hamster epididymis were investigated with conventional and lectin histochemistry. A zone of the caput
epididymis, with particular histochemical characteristics, has been differentiated. β-Elimination in combination with lectins
was used to establish the presence and distribution of N- and O-linked glycoconjugates. The epithelium, spermatozoa and the
intertubular matrix were rich in glycoconjugates. The Golgi apparatus and stereocilia of the principal cells were intensely
positive with HPA, PNA and SBA lectins. β-limination indicated that these cells contained abundant O-linked glycoconjugates.
Apical and clear cells presented a common lectin affinity; their reactivities towards WGA and UEA-I were very positive. These
cells probably contain abundant N-glycoconjugates. The spermatozoa were stained by periodic acid-Schiff (PAS) and by all the
lectins (especially in the acrosome), except by those with an affinity for α-l-fucosyl residues; the most intense reaction was found with HPA, WGA, PNA and SBA. Changes in the sperm lectin binding along
the ductus were observed: sperm flagellum abruptly acquired WGA and PNA labelling from the posterior caput, and HPA reactivity
was negative only in the zone between the caput and the corpus. |
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