Long term production of acute-phase proteins by adult rat hepatocytes co-cultured with another liver cell type in serum-free medium |
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Authors: | A Guillouzo F Delers B Clement N Bernard R Engler |
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Institution: | 1. Unité de Recherches Hépatologiques U 49 de l''INSERM Hôpital Pontchaillou, 35011 Rennes, France;2. Laboratoire des protéines de la réaction inflammatoire UER Biomédicale des Saint-Pères, 45, rue des St Pères, 75006 Paris, France |
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Abstract: | Three acute-phase proteins, haptoglobin, alpha 2-macroglobulin and hemopexin, as well as albumin, have been measured daily in the hydrocortisone-supplemented serum-free medium of pure and mixed cultures of adult rat hepatocytes for 5 and 20 days respectively. Whereas plasma protein production rapidly declined in pure culture, it remained relatively stable when hepatocytes were co-cultured with rat liver epithelial cells. In the latter cultures, an early stimulation of albumin and alpha 2-macroglobulin secretion was observed. In addition, four other plasma proteins, fibrinogen, alpha 1-acute-phase protein, alpha 1-acid glycoprotein and alpha 1-antitrypsin were shown by immunodiffusion to still be produced by day 20 of co-culture. These results suggest that hepatocyte co-cultures represent a suitable model for studying the mechanism which controls synthesis of plasma proteins, including acute-phase proteins by liver cells. |
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