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The synthesis of C-19 steroids by guinea-pig adrenal homogenates
Authors:E S Lwowski  D W Killinger
Affiliation:Departments of Physiology and Medicine and the Wellesley Hospital, University of Toronto, Toronto, Ontario, Canada
Abstract:Guinea-pig adrenal homogenates were incubated with [3H]-pregnenolone as substrate. There was conversion to progesterone, cortisol, cortisone, 11β-hydroxyandrostenedione (11βOHA), androstenedione, adrenosterone and testosterone. Dehydroepiandrosterone could not be isolated. Of the total amount of substrate, 3.26% was converted to 11βOHA, and 4.08% was converted to cortisol. In an incubation using [14C]-cortisol as substrate, 2.34% conversion to 11βOHA was obtained. When adrenal homogenates were incubated with both [3H]-pregnenolone and [14C]-cortisol, the 3H/14C ratio in isolated 11βOHA was 3–4 fold higher than the 3H/14C ratio in isolated cortisol. These results indicate that cortisol is not the major precursor of 11βOHA in guinea-pig adrenal. It is likely that most of 11βOHA is formed from androstenedione.
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