Plant regeneration from callus cultures of <Emphasis Type="Italic">Maclura tinctoria</Emphasis>, an endangered woody species |
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Authors: | Guilherme?Augusto?Canella?Gomes Email author" target="_blank">Renato?PaivaEmail author Patrícia?Duarte?de?Oliveira?Paiva Edson?José?Artiaga?de?Santiago |
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Institution: | (1) Laboratório de Cultura de Tecidos, Setor de Fisiologia Vegetal do Depto, de Biologia, Universidade Federal de Lavras (UFLA), 37200-000 Lavras-MG, Brazil |
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Abstract: | Summary Some native species produce seeds with a low frequency of germination accompanied with a period of dormancy. These features
make it difficult to produce new phenotypes through sexual propagation. Maclura tinctoria has been considered an endangered species due to extensive use of its wood and low frequency of seed germination. The objective
of the present study is to establish an in vitro propagation system for this species. Organogenic friable callus formation from nodal segments has been obtained using woody
plant medium (WPM) supplemented with 10.74 μM 1-naphthaleneacetic acid (NAA)+4.43 μM 6-benzylaminopurine (BA). Results indicate that the highest frequency of shoot formation is observed when WPM supplemented
with 4.03 μM NAA+4.43 BA is used. For root formation, the use of WPM medium (pH adjusted to 7.0) supplemented with 23.62 μM indole-3-butyric acid (IBA) and 4.7gl−1 activated charcoal is recommended. For acelimatization, subjecting rooted plantlets to 70%, 50%, and 30% mesh screen, each
successively for a period of 7 d, has resulted in 97% plantlet survival. |
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Keywords: | Maclura tinctoria native woody plant micropropagation callus |
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