Amino acid composition and terminal residues of aspartate aminotransferase from ox heart |
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Authors: | G Marino V Scardi and R Zito |
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Institution: | Laboratorio di Chimica delle Fermentazioni, Istituto di Chimica Organica dell''Università di Napoli, Naples, and Istituto Regina Elena, Rome, Italy |
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Abstract: | 1. The amino acid composition of highly purified aspartate aminotransferase from ox heart was determined. 2. Alanine is the only N-terminal residue. 3. Leucine was identified as the only C-terminal residue. 4. No disulphide bridges are present in the enzyme molecule. 5. The thiol groups are not equally accessible, the accessibility being comparatively easier in the apoenzyme molecule. |
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