|
|||||
|
|
DNA degradation at elevated temperatures after plasmid amplification in amino acid-starved Escherichia coli cells |
|
| Authors: | Peter Neubauer Borys Wróbel Grzegorz W?grzyn |
| Institution: | (1) Martin Luther University of Halle-Wittenberg, Institute of Biotechnology, Weinbergweg 16a, 06120 Halle, Germany;(2) Department of Molecular Biology, Laboratory of Molecular Genetics, University of Gda sk, K adki 24, 80-822 Gdask, Poland |
| Abstract: | Summary Amino acid starvation of Escherichia coli relA mutants may be used as a method for efficient plasmid DNA amplification. Here we demonstrate DNA degradation which occurs at elevated temperatures (42–43°C) after plasmid amplification in amino acid-starved bacteria. These results may explain the previously described low efficiency of plasmid DNA amplification at elevated temperatures. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |