Spin label and microcalorimetric studies of the interaction of DNA with unilamellar phosphatidylcholine liposomes |
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Authors: | L Vojcíková E Svajdlenka P Balgavy |
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Affiliation: | Faculty of Sciences, P. J. Safárik University, Kosice, Czechoslovakia. |
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Abstract: | High-molecular DNA from chicken erythrocytes interacts with 1,2-dipalmitoylphosphatidylcholine in unilamellar liposomes, both in the presence and absence of Mg2+ ions. This interaction results in a phase separation in liposome membranes. The new phase induced by DNA and Mg2+ has a higher gel-liquid crystal phase transition temperature as measured by microcalorimetry. In the liquid crystalline state, the 16- and 5-doxyl stearic acid spin labels indicate changed local bilayer properties at the label position in the new phase. |
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