Immunocytochemical localization of ecdysteroids in the life history stages of Schistosoma mansoni |
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| Authors: | P F Basch |
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| Affiliation: | 1. Institute of Neuroscience, University of Oregon, Eugene, OR, USA;2. Department of Genome Sciences, University of Washington, Seattle, WA, USA;1. Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan;2. Biogen, Cambridge, MA, USA;3. Center for Neuropsychiatric Research, National Health Research Institutes, Miaoli, Taiwan;4. Department of Psychiatry, College of Medicine and National Taiwan University Hospital, Taipei, Taiwan;5. Marcus Institute for Aging Research and Harvard Medical School, Boston, MA, USA;6. Beth Israel Deaconess Medical Center, Boston, MA, USA;7. Harvard School of Public Health, Boston, MA, USA;8. Broad Institute of MIT and Harvard, Cambridge, MA, USA;9. Genomics Research Center, Academia Sinica, Taipei, Taiwan;10. Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan;11. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;12. Biomedical Translation Research Center, Academia Sinica, Taipei, Taiwan;13. Taiwan Biobank, Academia Sinica, Taipei, Taiwan;14. Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan;15. Graduate Institute of Environmental Science, China Medical University, Taichung, Taiwan;16. Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA;17. Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, USA;18. Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, MA, USA;19. Department of Medicine, Harvard Medical School, Boston, MA, USA;20. Department of Public Health & Medical Humanities, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan;21. Institute of Behavioral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan;1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming, Yunnan 650201, China;2. Graduate University of the Chinese Academy of Science, Beijing, 100049, China;3. Key Laboratory for Aging and Regenerative Medicine, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, China;4. The Center for Precision Medicine of First Affiliated Hospital, Biomedical Translational Research Institute, Jinan University, Guangzhou, Guangdong 510632, China;1. Department of Biology, Stanford University, Stanford, CA, USA;2. Department of Bioengineering, Stanford University, Stanford, CA, USA |
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| Abstract: | Sporocysts, cercariae and adults of S. mansoni exhibit focal immunoreactivity against anti-ecdysone serum in an indirect immunofluorescence assay. In adult males immunoreactivity is limited to cell bodies and linear connections in the parenchyma surrounding the intestinal caeca. In both unisexual and paired mature females part of the lining of the ootype is reactive, especially near the entrance of the vitelline duct; this demonstrates that females can make ecdysteroids without mal contact. Adult worms cultured completely in vitro show a similar pattern of reactivity. Immunoreactivity is strong in cercariae, but is essentially absent in miracidia. |
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