用EST-SSR标记对茶树种质资源的研究

采用16对EST-SSR引物对42份茶树品种资源进行了分析。在这些引物中, 有13对可扩增出清晰条带, 其中10对引物具有多态性, 占76.9%。各多态性引物的PIC值变化范围为0.522~0.866, 平均PIC值为0.730。10对多态性引物共检测到84种等位基因型和74个等位变异, 每对引物可检测到基因型和等位基因数分别为4~12和3~10种。42份供试材料间遗传距离为0.074~0.667, 平均遗传距离为0.363, 说明本实验所测试的材料具有较广的遗传变异。在相似系数为0.55的水平可将这些材料聚为3类, 多数材料包含在第一类中。本研究表明, 利用EST-SSR标记进行茶树资源评价是有效的。

Studies on tea plants (Camellia sinensis) germplasms using EST- SSR marker

Forty two tea varieties were analyzed by using 16 SSR primer sets derived from tea ESTs in this study, and 13 of the primer sets produced clear bands and 10 of them showed polymorphism, accounting for 76.9%. The PIC (polymor- phism information content) for each polymorphic primer set varied from 0.522 to 0.866, with a average about 0.73. Totally 84 Genotypes and 74 alleles were detected in all materials by 10 polymorphic markers, with the range from 4 to 12 and from 3 to 10 for each polymorphic primer set, respectively. The genetic distance among 42 tea varieties varied from 0.074 to 0.667, averagely 0.363, suggesting that the materials used in the experiment possess a broad genetic variation. Based on the similarity coefficient about 0.55, all the tea varieties tested could be classified into 3 groups and most of them were in first group. The results of this study proved that the EST-SSR marker is very effective in evaluation of tea germplasms.