石油降解细菌降解条件优化研究

采用富集培养法从工业油污土壤中分离到1株能以石油为惟一碳源而生长的细菌菌株,采用正交设计实验对该菌株的降解条件进行了初步研究。结果表明,最佳降解条件为NH_4Cl 4.0 g/LL,K_2HPO_4 1.5 g/L,pH 8.0,NaCl 15.0 g/L。在最佳条件下,浓度为1 mL/L的原油可在4 d内降解50%以上。

Degradation Conditions Optimization of Petroleum-Degradable Bacteria X15

Enrichment culture method was adopted to isolate a bacterial strain from industrial oil polluted soil that took petroleum as sole carbon source to grow,and orthogonal design was used to initially study and test the degradation conditions of the strain.The results showed that the optimized conditions were:NH_4Cl 4.0 g/L,K_2HPO_4 2.5 g/L, pH 7.0,and NaCl 6.0 g/L.Under these optimized conditions,petroleum concentration at 1 ml/L was degraded by over 50% within 4 days.