一株寒地高效解无机磷细菌的分离鉴定及拮抗作用

【目的】从北方寒地种植的不同农作物根际土壤中分离高效解磷的细菌,为微生物制剂和磷肥的开发提供适于本地区的优良菌种。【方法】通过初筛和复筛从26株解磷菌中筛选获得一株高效解磷细菌,对其进行生理生化和分子生物学鉴定,同时采用钼蓝比色法测定解磷能力。采用平板对峙法测定拮抗植物病原菌能力。【结果】通过筛选后获得的菌株B51-7经鉴定为伯克霍尔德菌属。菌株在发酵液中可溶性磷含量最高达到832.74 mg/L,同时具有很强的广谱抑菌作用,抑菌率最高为89.71%,可以显著促进水稻生长。【结论】菌株B51-7是一株具有生物防治作用的高效解磷细菌,可应用于生物菌肥和生防制剂中。

Identification and antagonism activity of an inorganic phosphorus-dissolving bacterial strain isolated from cold region

Objective] In order to find strains suitable for the development of biocontrol agents and biophosphate fertilizer, bacteria with high phosphorus-dissolving efficiency were isolated from the rhizosphere soils of different crops planted in the cold region of north China.Methods] A highly effective phosphorous-dissolving bacterium was obtained from 26 bacterial strains with phosphorous-dissolving activity through preliminary and second screening. The bacterial strain was identified through physiological, biochemical and molecular biological methods, and its phosphorus-dissolving activity was tested by molybdenum blue colorimetry method. Meanwhile, the antagonism activity to several plant pathogens was determined by plate confrontation method.Results] Strain B51-7 was obtained by the screening and identified as Burkholderia sp.. The soluble phosphorus content of the strain B51-7 in the fermentation broth reached 832.7 mg/L. Meanwhile, this strain had a broad-spectrum antifungal activity with the highest antagonism rate of 89.71%. A pot experiment showed that the growth of rice was significantly promoted by inoculation of strain B51-7.Conclusion] Strain B51-7 was a highly effective phosphate-dissolving bacterium with biological control effect, and could be applied for making biological fertilizers and biocontrol agents.