Enzymic acylation of 6-aminopenicillanic acid |
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| Authors: | B McDougall P Dunnill MD Lilly |
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| Institution: | Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK |
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| Abstract: | The enzymic synthesis of benzylpenicillin from 6-aminopenicillanic acid in the presence of poly (ethylene glycol) has been studied. With equimolar initial concentrations (20 mM) of 6-aminopenicillanic acid and phenylacetic acid a 60% conversion to benzylpenicillin can be achieved at 10°C and pH 5.2 in the presence of 45% (w/v) poly(ethylene glycol). Under these conditions the lactam ring of the benzylpenicillin and 6-aminopenicillanic acid and the enzyme, penicillin acylase (penicillin amidase, penicillin amidohydrolase, EC 3.5.1.11), were more stable than in the absence of the polyol. |
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| Keywords: | Penicillin 6-aminopenicillanic acid acylation poly(ethylene glycol) |
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