天麻素对CUS大鼠抑郁样行为和海马BDNF/GDNF水平的影响

目的:探讨天麻素对慢性不可预见应激(CUS)大鼠抑郁样行为的改善作用及对海马脑源性神经营养因子(BDNF)及胶质原纤维酸性蛋白(GDNF)表达的影响。方法:将64只SD大鼠随机分为对照组(Sham),Sham+天麻素低、中、高剂量组,模型组(CUS),模型(CUS)+天麻素低、中、高剂量组,每组8只。对照组每天腹腔注射生理盐水(1 m L/kg),连续14天;Sham+天麻素低、中、高剂量组每天腹腔注射不同剂量的天麻素(50、100或者200 mg/kg),连续14天;模型组接受CUS造模,并且在造模结束后每天腹腔注射生理盐水(1 m L/kg),连续14天;模型+天麻素低、中、高剂量组在CUS造模结束后每天腹腔注射不同剂量的天麻素(50、100或者200 mg/kg),持续14天。随后,通过糖水偏好实验和强迫游泳实验检测各组大鼠的抑郁样行为,在行为学检测结束后处死大鼠,通过Elisa检测海马GFAP和BDNF的表达情况。结果:(1)慢性不可预见应激(CUS)可以导致明显的抑郁样行为,包括糖水偏好减少(P0.05)和强迫游泳不动时间增加(P0.01),CUS组大鼠海马GFAP和BDNF水平下降(P0.01)。(2)一定剂量(100和200 mg/Kg)天麻素干预可以缓解CUS大鼠的抑郁行为,CUS与CUS+GAS(M)组(P0.05)以及CUS与CUS+GAS(H)组之间(P0.01)存在显著性差异。(3)中高剂量的天麻素可以恢复CUS大鼠海马的BDNF和GDNF水平,CUS与CUS+GAS(M)组(P0.05)以及CUS与CUS+GAS(H)组之间(P0.05)的BDNF和GDNF水平存在显著性差异。结论:天麻素可以缓解CUS模型大鼠抑郁样行为,恢复CUS模型大鼠海马的BDNF和GDNF水平。

The Influence of Gastrodin on Depressive-like Behavior on CUS Rats and the Expression of BDNF/GDNF on the Hippocampus

ABSTRACT Objective: To investigate the antidepressant-like effect on CUS rats and the effect on hippocampus BDNF/GDNF expression gastrodin. Methods: SD rats were randomly assigned to the following 8 groups (n = 8 for each groups): Sham, Sham + GAS (L), Sham + GAS (M), Sham + GAS (H), CUS, CUS + GAS (L), CUS + GAS (M) and CUS + GAS (H). The Sham group received only saline, and the Sham + GAS (L, M or H) group received GAS (50, 100 or 200 mg/kg daily). The rats in the CUS group were subjected to CUS, and then received saline as a vehicle for 14 days (1 mL/kg daily). The CUS + GAS (L, M or H) group also experienced CUS, but they received GAS (50, 100 or 200 mg/kg daily) for 14 days. After 14 days of treatment with GAS or vehicle, rats were experienced sucrose preference test and forced swim test, then sacrificed and the expressions of GFAP and BDNF in the hippocampus were determined by Elisa. Results: (1) The rat in CUS group showed significant more immobility time in forced swim test and decreased sucrose preference; (2) GAS administration (100 and 200 mg/kg daily) reversed depressive-like behaviors in rats exposed to CUS paradigm; (3) GAS administration restored the expression of GFAP and BDNF in the hippocampus of CUS rat. Conclusion: GAS administration (100 and 200 mg/kg daily) reversed depressive-like behaviors in rats exposed to CUS paradigm and restored the expression of GFAP and BDNF in the hippocampus.