人参皂苷单体Rh2对人鼻咽癌CNE-2S细胞增殖及凋亡的影响

目的:探讨人参皂苷单体Rh2对人鼻咽癌CNE-2S细胞增殖及凋亡的影响。方法:将生长在对数期的人鼻咽癌CNE-2S细胞分为空白对照组、阴性对照组和实验组。对照组常规培养,阴性对照组采用含有DMSO的培养液培养,实验组在对照组细胞的基础上加入不同浓度人参皂苷单体Rh2处理。采用MTT法测定细胞增殖,PI单染流式细胞术分析各时期细胞所占百分比,Annexin V-PI双染流式细胞仪检测细胞的凋亡情况。结果:与阴性对照组相比,实验组各浓度下的Rh2对CNE-2S细胞均具有显著的增殖抑制作用(P0.05),且随着Rh2浓度的增加而呈现增强的趋势,其中浓度为12.5 mg·L-1 Rh2增值抑制率最低,浓度为100 mg·L-1Rh2增值抑制率最高。不同浓度人参皂苷单体Rh2 G0/G1期细胞分布显著高于阴性对照组(P0.001),且G2/M、S期细胞比例显著低于阴性对照组(P0.01),且随着人参皂苷单体Rh2浓度的增加作用呈现增强的趋势(P0.05);不同浓度的Rh2单体作用24h,CNE-2S细胞早期、晚期凋亡率及总凋亡率均较阴性对照组明显增高(P0.001),并且在Rh2单体浓度为100 mg·L-1时,凋亡率最高。结论:人参皂苷单体Rh2对人鼻咽癌CNE-2S细胞增殖及凋亡具有显著的影响,并且可能对单体Rh2的浓度存在依懒性。

Influence of Ginsenoside Monomer Rh2 on the Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma Cell Line CNE-2S

ABSTRACT Objective: To explore the influence of ginsenoside monomer Rh2 on CNE-2S cell proliferation and apoptosis of human nasopharyngeal carcinoma. Methods: Human nasopharyngeal carcinoma CNE-2S cells growed in the logarithmic phase were divided into blank control group, negative control group and experimental group. The control group were cultured routinely, the negative control group were cultured with DMSO, and the experimental group were added ginsenoside monomer Rh2 on the basis of the control groups. MTT method was used to measure cell proliferation. PI single staining flow cytometry was used to analyze cell percentage in each period of the cell cycle. Effects of monomer Rh2 on CNE-2S cell proliferation of human nasopharyngeal carcinoma were observed. AnnexinV-PI double staining flow cytometry was used to detect cell apoptosis. Results: Compared with the negative control group, the experimental group and the levels of Rh2 in CNE-2S cells significantly inhibited the proliferation (P < 0.05), and with the increase of Rh2 concentration and showed a increasing trend, the concentration of 12.5 mg?L^-1 Rh2 proliferation inhibition rate is lowest, the concentration of 100 mg?L^-1 Rh2 added the highest inhibition rate. The distribution of single Rh2 G0/G1 cells with different concentrations of ginsenoside was significantly higher than that of the negative control group (P<0.001), and G2/M, the percentage of cells in S phase was significantly lower than that of the negative control group (P<0.01), and with ginsenoside Rh2 concentration increased has increased (P < 0.05); The early and late apoptosis rate and total apoptosis rate of CNE-2S cells with different concentrations of 24h were significantly higher (P<0.001) than that of negative control group, and the highest apoptosis rate was 100 mg?L^-1 when the concentration of Rh2 was Rh2. Conclusion: Ginsenoside monomer Rh2 has significant influence on CNE-2S cell proliferation and apoptosis of human nasopharyngeal carcinoma, and may be lazy on concentration of monomer Rh2.