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IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用机制研究
引用本文:张海佳,沙素梅,徐 斌,王 虎,许 冰,王国栋,蒋铭佐,聂勇战,吴开春,李孟彬.IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用机制研究[J].现代生物医学进展,2017,17(17):3211-3215.
作者姓名:张海佳  沙素梅  徐 斌  王 虎  许 冰  王国栋  蒋铭佐  聂勇战  吴开春  李孟彬
作者单位:第四军医大学西京消化病医院 陕西 西安 710032;第四军医大学西京消化病医院 陕西 西安 710032;中国人民解放军第174医院 福建 厦门 361000
基金项目:国家自然科学基金项目(81500423,81370504,81502082,81600443)
摘    要:目的:评价IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用及其可能机制。方法:选择野生型及IL-17基因敲除的SPF级C57BL/6小鼠并随机分成4组,分别给予不同的处理:(1)野生小鼠+单纯蒸馏水灌胃处理组;(2)野生小鼠+E.coli LF82(1×109/CFU/只)灌胃10天处理组;(3)IL-17敲除小鼠+单纯蒸馏水灌胃处理组;(4)IL-17敲除小鼠+E.coli LF82灌胃10天处理组。从以下5个方面评价各组小鼠的炎症反应和IL-17水平:(1)组织病理评分评估炎症反应严重程度;(2)透射电镜下观察结肠上皮细胞的超微结构;(3)免疫组织化学检测结肠分泌的IL-17;(4)PCR检测小鼠结肠中IL-17m RNA表达;(5)ELISA检测结肠组织中IL-17的含量。结果:定植了E.coli LF82的IL-17敲除小鼠肠道炎症程度和超微结构损伤较野生型小鼠更加严重(P0.05)。与未经E.coli LF82处理组相比,定植了E.coli LF82的野生小鼠肠道中IL-17m RNA和IL-17含量明显升高(P0.05)。结论:IL-17在E.coli LF82在黏附侵袭结肠粘膜过程中的保护作用,IL-17是针对AIEC菌株E.coli LF82免疫的重要效应物,而结肠局部分泌增加的IL-17会改善感染的结果。

关 键 词:炎症性肠病  大肠杆菌LF82  白细胞介素17
收稿时间:2017/1/4 0:00:00
修稿时间:2017/1/26 0:00:00

The Role and Mechanism of IL-17 in the Colon Infected by Adherent Invasive Escherichia coli LF82
Abstract:ABSTRACT Objective: To assess the role of IL-17 in the colon of mice infected by adherent-invasive Escherichia coli (AIEC) strain E.coli LF82. Methods: The wild-type (WT) mice and IL-17KO (IL-17 knockout) mice placed in specific pathogen free (SPF) housing were randomly divided into 4 groups: (1) WT mice + distilled water; (2) WT mice + E. coli LF82 by oral gavage for 10 days; (3) IL-17KO mice + distilled water; (4) IL-17KO mice + E.coli LF82 by oral gavage for 10 days. Four aspects of the treatment response were evaluated in each group: (1) histological score; (2) transmission electron microscope (TEM) to explore the ultrastructure; (3) the secretion of IL-17 detected by immune histochemistry; (4) the relative quantitative analysis of IL-17 in the colon detected by RT-PCR; (5) the quantitative analysis of IL-17F in the colon detected by ELISA. Results: Compared with non-treatment group, the expression of IL-17 mRNA and the activity of IL-17 were significantly higher than that in WT mice (P<0.05). After E. coli LF82 treatment, compared with WT mice, the histological score and the ultrastructure in IL-17KO mice improved significantly (P<0.05). Conclusion: IL-17 protects colonic epithelial integrity in the colon infected by adherent invasive Escherichia coli LF82. IL-17 is an important effector of immunity to AIEC strain E.coli LF82, suggestting that an increased local production of IL-17 would improve the outcome of infection.
Keywords:Inflammatory bowel disease  Escherichia coli LF82  IL-17
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