脂肪干细胞的诱导分化及其来源的研究

目的:通过组织块培养法得到脂肪干细胞(adipose-derived stem cells,ADSCs),探讨其诱导分化潜能,并初步研究ADSCs的来源。方法:用脂肪组织块培养法培养原代人ADSCs。第三代ADSCs进行成脂和成骨诱导分化,分别用油红O和茜素红S染色进行鉴定。脂肪组织块培养七天后取脂肪组织进行Hematoxylin-eosin Staining(HE)染色观察ADSCs组织分布。结果:用脂肪组织块培养法成功培养出原代人ADSCs。ADSCs传代到第8代,依然保持着良好的增殖能力和细胞形态。ADSCs能成功诱导成脂肪细胞和骨细胞。通过对培养七天后的脂肪组织块进行HE染色,发现ADSCs主要分布在脂肪组织的间质血管和结缔组织周围。结论:用脂肪组织块培养出来的ADSCs具有成脂和成骨分化的潜能。ADSCs主要定位于间质血管和结缔组织周围。

Research on the Induced Differentiation of Adipose Derived Stem Cells and Its Origin

ABSTRACT Objective: To investigate the induction and differentiation potential of ADSCs by tissue culture method, and to prelim- inary study on the origin of ADSCs. Methods: Using adipose tissue culture method to culture human ADSCs. The third generation of ADSCs for the adipogenic and osteogenesis differentiation, and staining by oil red O and alizarin red S. HE staining was performed after the seventh day culture of adipose tissue. Results: The primary human ADSCs were successfully cultured with adipose tissue culture method. ADSCs cultured to the eighth generation, still maintained a good proliferation ability and cell morphology. ADSCs can be success- fully induced into adipose cells and bone cells. ADSCs were mainly distributed around the mesenchymal vascular and connective tissue, by HE staining of adipose tissue after seven days of culture. Conclusion: The cells that were cultured with adipose tissue have the poten- tial to adipogenic and osteogenesis differentiation. The ADSCs were mainly distributed around the mesenchymal vascular and connective tissue.