骨髓间充质干细胞移植对脑梗死大鼠神经功能恢复的影响

目的:观察骨髓间充质干细胞(BMSC)移植对脑梗死大鼠神经功能恢复的影响,并对其相关机制进行探讨。方法:90只大鼠随机分为3组:假手术组、对照组、BMSC移植组,每组30只。对照组和BMSC移植组建立大鼠大脑中动脉阻塞(MCAO)模型,假手术组只需要分离大鼠颈部组织,而不造MCAO模型。BMSC移植组在MCAO模型术后1天经尾静脉注射1 mL/3×10~6 BMSC,对照组注射同剂量的生理盐水,于MCAO术后1 d、3 d、7 d、14 d、21 d、28 d、35 d、42 d、49 d分别对各组大鼠进行神经功能评分(mNSS),术后2个月对BMSC移植组及对照组大鼠脑组织进行免疫组化染色,检测MAP2、TUJ1、Ⅷ因子、GFAP的表达情况。结果:在治疗后的第7天至第35天,BMSC移植组mNSS均显著低于对照组(P0.05)。术后2个月,BMSC移植组MAP2、TUJ1、Ⅷ因子表达量显著高于对照组,而GFAP表达量显著低于于BMSC对照组(P0.01)。结论:BMSC移植可以促进脑梗死神经功能的恢复。

Research of Bone Marrow Mesenchymal Stem Cell Transplantation for Rat Cerebral Infarction Recoveryof Neurological Function

ABSTRACT Objective: To investigate the effect of BMSC transplantation on the recovery of neurological function in rats with cerebral infarction, and to explore the related mechanism. Methods: 90 rats were randomly divided into 3 groups: sham operation group, control group, BMSC transplantation group, 30 rats in each group. The control group and BMSC transplantation group established middle cerebral artery occlusion (MCAO) model, the sham operation group only need to separate the cervical tissue of rats, and MCAO model in the MCAO model operation. After 1 days of BMSC transplantation group by intravenous injection of 1 mL 3×10⁶ BMSC, the control group was injected with the same dose of NS in MCAO after 1 D, 3 D, 7 d, 14 d, 21 d, 28 d, 35 d, 42 d, 49 D respectively, the neurological function score of rats (mNSS), after 2 months of transplantation BMSC group and control group of brain tissue for immunohistochemical staining, detection of MAP2, TUJ1, VIII factor, the expression of GFAP. Results: In seventh to thirty-fifth days after treatment, BMSC mNSS transplantation group were significantly lower than the control group (P < 0.05). 2 months after BMSC transplantation group MAP2, TUJ1, VIII expression level was significantly higher than the control group, while the control group, the expression of GFAP was significantly higher than that of BMSC group (P < 0.01). Conclusion: BMSC transplantation in order to promote the recovery of neurological function in cerebral infarction.