激光共聚焦显微镜在肿瘤免疫治疗检测中的应用

目的:通过激光共聚焦显微镜对肿瘤生物治疗后患者的外周血淋巴细胞进行亚群计数,为生物治疗后外周血淋巴细胞无法分群的肿瘤患者提供新的监测免疫功能状态的方法。方法:收集35例肿瘤生物治疗后患者的外周血标本,通过激光共聚焦显微镜和流式细胞仪两种方法分别对患者外周血淋巴细胞亚群进行分类计数。结果:流式细胞仪和激光共聚焦显微镜同时分类计数的患者外周血细胞标本30例,两种方法在检测CD3、CD3~+/CD4~+、CD3~+/CD8~+、CD3-/CD16~+56~+、CD3-/CD19~+细胞时均无统计学差异(P值0.05);5例流式细胞仪无法将患者外周血淋巴细胞分群的样本,通过激光共聚焦显微镜可以进行分类计数。结论:激光共聚焦显微镜亦可以用于外周血淋巴细胞的分类计数。

Application of Laser Scanning Confocal Microscope in the Detection and Immunotherapy of Tumors

ABSTRACT Objective: Through count the sub-groups of peripheral blood lymphocyte of tumor patients after biological treatment by laser scanning confocal microscope (LSCM), a new method developed to test immune state of unable to object tumor patients. Methods: Collected 35 Peripheral Blood (PB) samples of tumor patients after biological treatment, and counted the sub-groups of their peripheral blood lymphocyte by LSCM and flow cytometry (FCM). Results: 30 PB samples were counted by both LSCM and FCM, and there was no significant difference between LSCM and FCM. 5 PB samples which can not be counted by FSM, can be counted by LSCM. Conclusion: LSCM can be used for counting the sub-groups of peripheral blood lymphocyte.