| CRISPR/Cas9基因编辑技术在丝状真菌中的应用 |
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| 作者姓名： | 于鲲 薛佳琪 王进宽 余永涛 |
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| 作者单位： | 1.宁夏大学农学院，银川 750021;2.固原市原州区张易镇畜牧兽医工作站，宁夏 固原 756022 |
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| 基金项目： | 国家自然科学基金项目(31960348) |
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| 摘 要： | 随着对丝状真菌基因水平研究的不断深入，CRISPR/Cas9技术作为先进的基因编辑技术，已被广泛应用于丝状真菌的基因编辑。探究了CRISPR/Cas9系统在不同丝状真菌中的应用情况，主要从sgRNA的构建与表达、Cas9蛋白的改造与表达、不同的DNA双链断裂修复（DNA double-strand break，DSB）方式等方面进行概述，并对编辑效率、脱靶效应进行总结，旨在为今后丝状真菌中CRISPR/Cas9系统的构建及改良提供思路。
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| 关 键 词： | 丝状真菌 基因编辑 CRISPR/Cas9 |
| 收稿时间： | 2022-03-17 |
Research Progress on Application of CRISPR/Cas9 Gene Editing Technique in Filamentous Fungi |
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| Authors: | Kun YU Jiaqi XUE Jinkuan WANG Yongtao YU |
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| Institution: | 1.School of Agricuture Ningxia University，Yinchuan 750021，China;2.Animal Husbandry and Veterinary Workstation of Zhangyi Town，Yuanzhou District，Ningxia Guyuan 756022，China |
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| Abstract: | With the development of the research on filamentous fungi gene level， CRISPR/Cas9 technology is an advanced gene editing technology， which has been widely used in filamentous fungi gene editing. This article explored the application of the CRISPR/Cas9 system in different filamentous fungi. It included an overview of the construction and expression of sgRNA， the transformation and expression of Cas9 protein， and different DNA double-strand break （DSB） methods， and the editing efficiency and off-target effects were also summarized. It was expected to provided ideas for the construction and improvement of the CRISPR/Cas9 system in the future. |
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| Keywords: | filamentous fungi gene editing CRISPR/Cas9 |
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