An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2 |
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Authors: | Ying Tang Yiqin Wang Yuchang Li Huai Zhao Sen Zhang Ying Zhang Jing Li Yuehong Chen Xiaoyan Wu Chengfeng Qin Tao Jiang Xiaoping Kang |
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Institution: | a Department of Virology, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, Chinab State Key Laboratory of Pathogen and Biosecurity, AMMS, Beijing 100071, Chinac Beijing University of Chemical Technology, Beijing 100029, Chinad Technology Center of Erenhot Customs, Erenhot 011100, Chinae Lifereal Biotech. Inc., Hangzhou 311100, Chinaf Anhui Medical University, Hefei 230032, China |
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Abstract: | Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a novel coronavirus that causes the outbreak of coronavirus disease 2019 (COVID-19) (Li et al., 2020a). Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19 (Wu et al., 2020a; Zhu et al., 2020). Currently, a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection (Corman et al., 2020; Hussein et al., 2020; Ruhan et al., 2020; Veyer et al., 2020). Conventional qPCR involves virus inactivation, nucleic acid extraction, and qPCR amplification procedures. Therefore, the process is complicated, which usually takes longer than 2 h, and requires biosafety laboratories and professional staff. Thus, qPCR is not suitable for use in field or medical units. To reduce the operation steps, automatic integrated qPCR detection systems that combine nucleic acid extraction and qPCR amplification in a sealed cartridge were developed to detect viruses in clinical samples (Li et al., 2020b). However, the detection time is still longer than 1 h. Therefore, rapid nucleic acid detection systems are needed to further improve the detection efficiency. |
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