Cloning, sequencing and heterologous expression of a new chitinase gene, chi92, from Streptomyces olivaceoviridis ATCC 11238 |
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| Authors: | Haiming Li Hans Plattner Karl-Ludwig Schimz Michael Kieß Hans Diekmann Jochen Meens |
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| Affiliation: | (1) Institut für Mikrobiologie, Universität Hannover, Schneiderberg 50, 30167 Hannover, Germany;(2) Institut für Biotechnologie I, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany;(3) Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, 38124 Braunschweig, Germany;(4) Present address: toplab GmbH, 82152 Martinsried, Germany |
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| Abstract: | ![]()
A new chitinase gene, chi92, encoding the largest known chitinase from Streptomyces olivaceoviridisATCC 11238 was sequenced by means of different PCR-methods. The cloned gene was expressed in E. coliand the recombinant protein could be detected by Western-blot analysis. The multiplicity of chitinolytic enzymes of this strain is discussed. |
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| Keywords: | chitinase gene inverse PCR multiplicity RLM-RAGS Streptomyces olivaceoviridis |
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