The Additive Effect of Mesenchymal Stem Cells and Bone Morphogenetic Protein 2 on γ-Irradiated Bone Marrow in Mice |
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Authors: | Shuibing Liu Peizhen Hu Ying Hou Peng Li Xubo Li Qiong Tian |
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Institution: | (1) Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an, 710032, China;(2) Department of Pathology, Fourth Military Medical University, Xi’an, 710032, China; |
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Abstract: | Irradiation from γ-rays can cause severe damage to bone marrow and hematopoietic tissues. Presently, the most effective method
available to treat severe hematopoietic injury is a bone marrow transplant (BMT). Allogeneic BMT is a difficult technique
to perform due to the differences in human leukocyte antigen proteins between the donor and recipient, with acute graft-versus-host
disease being a major complication of the technique. This limits the widespread applicability of allogeneic BMT. To develop
a novel treatment for acute hematopoietic damage, we transplanted bone marrow derived mesenchymal stem cells (MSCs) into recipient
mice and treated them with recombinant human bone morphogenetic protein 2 (rhBMP2) to investigate whether MSCs and rhBMP2
could additively promote the restoration of hematopoietic function. MSCs are vital components of the hematopoietic microenvironment
that supports hematopoiesis, and bone morphogenic protein is a key factor in hematopoiesis. The 30-day survival rate as well
as the numbers of nucleated cells, bone marrow colony-forming unit-granulocyte macrophages, spleen colony-forming units and
peripheral blood cells were enumerated. The results showed that, after γ-irradiation and transplantation, MSCs and rhBMP2
additively promoted and improved hematopoietic restoration and function in vivo and in vitro. This additive effect of MSCs
and rhBMP2 may one day provide a novel means of treating acute hematopoietic damage. |
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