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Identification of Vibrio spp. in vibriosis Penaeus vannamei using developed monoclonal antibodies |
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| Authors: | Longyant Siwaporn Rukpratanporn Sombat Chaivisuthangkura Parin Suksawad Pornthip Srisuk Chutima Sithigorngul Weerawan Piyatiratitivorakul Somkiat Sithigorngul Paisarn |
| Institution: | a Department of Biology, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand b Center of Excellence for Marine Biotechnology at Chulalongkorn University, National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok 10330, Thailand c Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand |
| Abstract: | Immunohistochemical study using monoclonal antibodies specific to various shrimp viruses and Vibrio spp. was performed in shrimp samples died from unknown cause with symptoms of black stripes on lateral sides of cephalothorax or smoky body coloration. The positive results in muscular tissue were obtained with MAb VAL57 (specific to Vibrio spp.) and in hepatopancreas tissues with MAbs VVB158 (specific to V. vulnificus) and VPC701 (specific to V. parahaemolyticus). Twelve isolates of Vibrio spp. isolated from shrimp tissues were identified with various MAbs by dot blotting, biochemical tests and 16S rRNA gene. The results revealed three groups of V. vulnificus and one group of V. shilonii. All four groups of isolated Vibrio spp. were immunologically and biochemically different. None of the V. parahaemolyticus-like bacterium was isolated. The results demonstrated that the mortality in shrimp is accompanied by the presence of Vibrio spp. |
| Keywords: | Monoclonal antibody Penaeus vannamei Vibrio shilonii V parahaemolyticus V vulnificus Vibriosis |
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