鼓槌石斛的地理分布与菌根真菌区系组成的相关性

本文研究了兰科石斛属鼓槌石斛的地理分布与菌根真菌区系组成的相关性。采用克隆文库技术对云南省西双版纳和临沧市两个地区的8份野生鼓槌石斛根部样品进行了分析。结果表明,共从两个地区的鼓槌石斛根部获得了14个真菌的可操作分类单元（OTU）,主要隶属于胶膜菌科Tulasnellaceae和小纺锤菌目Atractiellales。分析鼓槌石斛对菌根真菌的专一性和偏好性发现,鼓槌石斛对菌根真菌专一性较低,每个植株都能同时与多种菌根真菌共生;不同地理分布的鼓槌石斛菌根真菌类群存在明显差异,两个地区的鼓槌石斛的菌根真菌中只有1个OTU是相同的,其余均不同。说明菌根真菌可能和鼓槌石斛的生态适应存在一定的相关性。

Correlation between distribution of Dendrobium chrysotoxum and mycorrhizal fungal community composition

The aim of this research is to illustrate the relationship between distribution of Dendrobium chrysotoxum (Orchidaceae) and mycorrhizal fungal community composition. Clone library technique was used to analyze mycorrhizal fungal community in 8 root samples of D. chrysotoxum collected from Xishuangbanna and Lincang of Yunnan Province. In total, 14 operational taxonomic units (OTUs) were obtained from the two sampling regions. These OTUs mainly belonged to Tulasnellaceae and Atractiellales. The mycorrhizal specificity and preference of D. chrysotoxum were further analyzed. The result suggested that D. chrysotoxum has low mycorrhizal specificity. Each plant can bring about symbiosis with more than one species of mycorrhizal fungi at the same time. Evident differences of mycorrhizal fungal community composition were detected in D. chrysotoxum samples collected from the two sampling regions. Only one OTU was shared in the two regions and the others were all quite different. Mycorrhizal fungi may correlate with ecological adaptation of D. chrysotoxum.