Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody |
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| Authors: | Frank W Symington Bondada Subbarao D E Mosier J Sprent |
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| Institution: | (1) Division of Research Immunology, Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, 19104 Philadelphia, Pennsylvania;(2) Laboratory of Immunology, Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania |
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| Abstract: | A DBA/1 B10.D2-specific monoclonal antibody (CY34) is described which defines a new murine B lymphocyte differentiation antigen designated Lyb-8.2. The ontogeny, strain distribution, and cell-surface density of the antigen were studied by radioimmunoassay and by fluorescence-activated cell sorter (FACS) analysis. Lyb-8.2 appears to be expressed on pre-B cells and on all mature B lymphocytes. Lyb-8.2 molecules immunoprecipitated from surface labeled B10.D2 spleen cells migrated in polyacrylamide gels with an apparent mol. wt. of 95000–105000 daltons and were bound by lentil lectin. The expression of Lyb-8.2 is controlled by a locus on chromosome 7 that is closely linked to Gpi-1 and RP-2. Added Lyb-8.2-specific antibody did not measurably impair B lymphocyte function in several in vitro systems studied. |
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