| 钙结合蛋白CR在坐骨神经压榨模型脊髓内的表达 |
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| 引用本文： | 阳历,郭桂平.钙结合蛋白CR在坐骨神经压榨模型脊髓内的表达[J].现代生物医学进展,2011,11(15):2955-2957. |
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| 作者姓名： | 阳历 郭桂平 |
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| 作者单位： | 1. 湖南浏阳市集里街道卫生院,湖南浏阳,410300
2. 中南大学湘雅公共卫生学院办公室,长沙410078;中南大学湘雅二医院临床心理科,长沙410008 |
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| 摘 要： | 目的:研究大鼠坐骨神经压榨模型的钙结合蛋白Calretinin(CR)在脊髓的时空变化规律,为探讨其在神经再生中的作用提供实验依据。方法:36只SD大鼠随机分为假手术对照组和坐骨神经压榨组,实验组压榨后分别存活1d到21d,免疫组化结合图像分析技术观察CR在脊髓分布和含量的变化。结果:在对照组,CR样阳性神经元主要分布于腰髓背角Ⅰ,Ⅱ层,Ⅲ～Ⅵ层只观察到一些散在分布的CR样阳性神经元,脊髓前角Ⅷ层和Ⅸ层内也可见一些多极的中间型阳性神经元。坐骨神经压榨1d后,分布于腰髓背角Ⅱ层内的CR样阳性神经元比对照组有轻微增加。3d后,CR样阳性神经元与对照组相比没有明显改变。7d后,CR样阳性神经元有轻微的减少;14d后,CR的表达显著下降;至21d,CR的表达有所恢复,但仍低于7d组。脊髓后角CR免疫阳性产物灰度值测定结果显示:术后14d后角CR表达最低,与对侧和对照组相比有统计学意义(P<0.05)。结论:坐骨神经压榨后CR表达变化呈现一定的时空模式,为进一步揭示CR在神经系统疾病中的作用提供实验依据。
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| 关 键 词： | 钙网蛋白 大鼠 脊髓 压榨 |
Expression of Calretinin in Spinal Cord of Rats Model of Sciatic Nerve Crush |
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| YANG Li,GUO Gui-ping.Expression of Calretinin in Spinal Cord of Rats Model of Sciatic Nerve Crush[J].Progress in Modern Biomedicine,2011,11(15):2955-2957. |
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| Authors: | YANG Li GUO Gui-ping |
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| Institution: | (2,3△) (1 The Jili street health center of Liuyang city,Hunan Liuyang,410300; 2 Administration Department of Xiangya Public Health College,Central South University,Changsha,410078; 3 Second Xiangya Hospital,Central South University,Changsha 410011,China) |
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| Abstract: | Objective:To study the spatio-temporal pattern of expression of calretinin(CR) in spinal cord and provide experimental evidence of its possible mechanism responsible for regeneration.Methods:A total of 36 SD rats were randomly divided into three groups: sham,control and sciatic crushed Animals in experimental group survived for 1,3,7,14 and 21 days respectively.The distribution and contents of CR in spinal cord was determined by immunohistochemistry and image-analysis.Results:In control animal,CR positive n... |
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| Keywords: | Calreticulin Rat Spinal cord Crush |
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